Expression of Recombinant Chinese Bovine Enterokinase Catalytic Subunit in P. pastoris and Its Purification and Characterization
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چکیده
منابع مشابه
Expression, Purification and Characterization of Human Recombinant Galectin 3 in Pichia pastoris
Background: Over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. Carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. Objectives: The purpose of this study was to express and purify recombinant human galec...
متن کاملCloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase.
Enterokinase (enteropeptidase) is a heterodimeric serine protease that is responsible for the physiological activation of trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)4-Lys. In this paper, we report the cloning and functional expression of a cDNA encoding the catalytic domain (light chain) of bovine enterokinase. The nucleotide sequen...
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expression, purification and characterization of human recombinant galectin 3 in pichia pastoris
background: over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. objectives: the purpose of this study was to express and purify recombinant human galect...
متن کاملP-191: Cloning and Expression of Recombinant Ovine FSH Hormone in Pichia Pastoris
Background: Follicle stimulating hormone is a heterodimeric protein composed of two subunits, α and ß, which are linked noncovalently. The hypophysial gonadotropin FSH plays an important role in the regulation of oocyte maturation, and a key component for growth of ovulator follicles in ewes. Materials and Methods: This study seeks to clone and express the ovine follicle stimulating hormone sub...
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ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2004
ISSN: 1745-7270
DOI: 10.1093/abbs/36.7.513